Activity-independent screening of secreted proteins using split GFP
نویسندگان
چکیده
منابع مشابه
Screening libraries to identify proteins with desired binding activities using a split-GFP reassembly assay.
Designer protein modules, which bind specifically to a desired target, have numerous potential applications. One approach to creating such proteins is to construct and screen libraries. Here we present a detailed description of using a split-GFP reassembly assay to screen libraries and identify proteins with novel binding properties. Attractive features of the split-GFP based screen are the abs...
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To understand the function of membrane proteins, it is imperative to know their topology. For such studies, a split green fluorescent protein (GFP) method is useful. GFP is barrel-shaped, consisting of 11 β-sheets. When the first ten β-sheets (GFP1-10) and the 11th β-sheet (GFP11) are expressed from separate genes they will self-assembly and reconstitute a fluorescent GFP protein. However, this...
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ژورنال
عنوان ژورنال: Journal of Biotechnology
سال: 2017
ISSN: 0168-1656
DOI: 10.1016/j.jbiotec.2017.05.024